It is an honor for Pakistan, that we have discovered a test for cancer diagnosis which is affordable by general public. The detail of this test has been published in internationally reputed Journals including European Journal of Gynecological Oncology and Pakistan Journal of Pharmaceutical Sciences. Lipids are carried in body fluids with the help of lipoproteins, chylomicrons transport of triglycerides from the intestine to all cells. Very low-density lipoproteins (VLDL) are involved in the transportation of triglycerides from the liver to other cells. Low-density lipoproteins (LDL) are responsible for the transport of cholesterol from liver to the cells and high density lipoproteins (HDL) are involved for the transport of cholesterol from cells to the liver. Chylomicrons and very low-density lipoproteins are rapidly catabolized. Thus triglycerides, cholesterol, LDL–cholesterol and HDL–cholesterol constitute plasma lipid profile. Different prospective studies were carried out by taking fasting blood samples from all the major four types of cancers: carcinomas (cancers of epithelial cells), sarcomas (cancers of connective tissues), lymphomas (cancers of lymphoid tissues), and leukemias (cancers of leukocytes and their precursors). None patient had a history of thyroid disease, diabetes or other major illness that could affect lipid metabolism. The patients were also not treated with any chemotherapy, radiation or surgery before the sample collection that may affect plasma lipid levels. The whole process of development of “Qadir test” was divided into different steps and stages, and published consequently. The protocols for performing the test were published in an Italian Journal “Pharmacologyonline”. The protocols include the estimation of plasma levels of triglycerides, total cholesterol, LDL-cholesterol and HDL-cholesterol using spectrophotometer. Chemical kits used for their estimations are easily available in medical/chemical markets. A research was published in “European Journal of Gynecological Oncology”. A total of 196 female individuals were included in that study for estimation of plasma lipid profile in patients of gynecologic carcinomas (cancers). Among the gynecologic cancers, major were patients of breast cancer and ovarian cancer. It was concluded from the study that plasma lipid levels, except HDL-cholesterol were raised in breast cancer and were decreased in other gynecologic cancers. HDL-cholesterol was decreased in all gynecologic cancers. As there was alteration in plasma lipid profile during gynecologic cancers, it might be helpful for diagnosis of the disease. Another study was performed on sarcoma patients and was published in “Pakistan Journal of Pharmaceutical Sciences”. 120 subjects were included in the project. This study showed that plasma lipid levels were decreased in sarcoma patients. Another study of lymphoma patients was completed with 180 individuals and was published in “Pakistan Pediatric Journal”. The study also concluded decrease in Plasma lipid levels lymphoma Patients. A prospective study was carried out in 180 individuals and was published in “Journal of Applied Pharmacy” a reputed Canadian Journal. This study concluded decreased in plasma lipid levels in leukemia patients. Finally, an article summarizing all the results of estimation of plasma lipid profile in different cancer types was published in “Pakistan Journal of Pharmaceutical Sciences”, which is internationally accepted journal and comparable with American and European Journals as it has Impact Factor and indexed in “Science citation index” published by “Institute of Scientific Information” (Organization that award ranking to Journals). As a summary, it was seen that plasma lipid profile (triglycerides, cholesterol, LDL–cholesterol and HDL–cholesterol) is changed in cancer patients. Therefore, the change in plasma lipid profile may be used as marker for diagnosis of cancer. Cholesterol and triglycerides have very important physiological role in cells. Cholesterol maintains functional as well as structural integrity of all biological membranes. It is also involved in the activity of membrane bound enzymes and is important for stabilization of the DNA helix. Cellular uptake and regulation of cholesterol is mediated by lipoprotein receptors especially located on the surface of the cells. For transport in plasma, triglycerides and cholesterol are packaged into lipoproteins, which are then taken up and degraded by cells to fulfill demands for cellular functions. Decreased concentrations of blood lipids in cancerous patients might be because of the carcinogenesis. As the carcinogenesis proceeds, more cells are growing; more lipids are being used for their proliferation. Therefore, lower concentration of blood lipids, as compared to the normal person, could be utilized as marker of malignancy. If the cancer is diagnosed in early stage, it can easily be controlled. There are many options for management of the disease including chemotherapy and surgery. Recent techniques, like radiofrequency ablation and gamma knife, are most promising for practical survivals of the patients. As a natural therapy, vitamin K, not only for the treatment of cancer but it should also be used for prevention of cancer. Vitamin K has been shown to safely suppress growth and invasion of human cancers. It exerts multiple effects on tumours, modifying growth factors and their receptor molecules in a way that makes them less able to stimulate tumour growth and progression. It freezes the cell cycle, blocking further replication. And it triggers programmed cell death by apoptosis through several distinctive mechanisms. Vitamin K inhibits DNA-building enzymes; block new blood vessel formation essential to support the rapid growth of tumour tissue; and disrupts crucial intracellular communications networks composed of microtubules, preventing the cells from proliferating in a coordinated fashion. Therefore, cancer may easily be managed by using those foods that contain vitamin K. Vitamin K is abundantly present in Green Leafy Vegetables (Kale), Natto (fermented soy), Spring onions (Scallions), Brussels Sprouts, Cabbage, Broccoli, Dairy (fermented), Prunes, Cucumbers and Dried basil. But early detection has been a problem due to costly diagnostic tests and unavailability of these tests in common laboratories. Thus, giving an affordable diagnostic cancer test will serve the humanity well.
